Transcription Profiling of Glycosyltransferases in Vitis vinifera Cultivars Following Smoke Exposure

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dc.contributor.authorvan der Hulst, L.
dc.contributor.authorHerliana, L.
dc.contributor.authorHassan, A.S.
dc.contributor.authorSchwerdt, J.G.
dc.contributor.authorShirley, N.
dc.contributor.authorFord, C.M.
dc.contributor.authorBurton, R.A.
dc.contributor.authorWilkinson, K.L.
dc.date.issued2025
dc.description.abstractSmoke taint is a fault that can occur in wines made from smoke-affected grapes. Smoke taint research has largely focused on chemical and sensory analysis and mitigation strategies, with limited attention to underlying molecular responses. We performed RNA sequencing on berries from Vitis vinifera cultivars Shiraz and Chardonnay after controlled smoke exposure. Significant transcript upregulation was observed in smoke-affected grapes, especially for heat shock proteins (HSP20s) and glycosyltransferases (GTs). Chardonnay showed upregulation of 15 GTs from GT1, GT8, GT32, GT47, and GT90, while Shiraz had only five upregulated GTs, primarily GT1 and GT8, suggesting cultivar-specific responses. Gene Ontology enrichment indicated activation of phenylpropanoid and xenobiotic response pathways. While prior studies focused on GT1, our results highlight potential roles for other GT families, particularly GT8. These findings expand our understanding of grapevine stress responses to smoke and identify new molecular targets to inform future strategies for managing smoke taint in grape production.
dc.description.statementofresponsibilityLieke van der Hulst, Lina Herliana, Ali Saleh Hassan, Julian G. Schwerdt, Neil Shirley, Christopher M. Ford, Rachel A. Burton, and Kerry L. Wilkinson
dc.identifier.citationJournal of Agricultural and Food Chemistry, 2025; 73(35):22046-22058
dc.identifier.doi10.1021/acs.jafc.5c06076
dc.identifier.issn0021-8561
dc.identifier.issn1520-5118
dc.identifier.orcidvan der Hulst, L. [0000-0002-7810-9431]
dc.identifier.orcidSchwerdt, J.G. [0000-0002-0476-031X]
dc.identifier.orcidShirley, N. [0000-0001-8114-9891]
dc.identifier.orcidFord, C.M. [0000-0003-1617-2977]
dc.identifier.orcidBurton, R.A. [0000-0002-0638-4709]
dc.identifier.orcidWilkinson, K.L. [0000-0001-6724-9837]
dc.identifier.urihttps://hdl.handle.net/2440/147832
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.relation.granthttp://purl.org/au-research/grants/arc/IC130100005
dc.rights© 2025 The Authors. Published by American Chemical Society.
dc.source.urihttps://doi.org/10.1021/acs.jafc.5c06076
dc.subjectsmoke taint; grapes; RNA-seq; GO pathway enrichment; glycosyltransferases; GT1; GT8; volatile phenols; Vitis vinifera; HSP20s; xenobiotics
dc.subject.meshVitis
dc.subject.meshFruit
dc.subject.meshGlycosyltransferases
dc.subject.meshPlant Proteins
dc.subject.meshGene Expression Profiling
dc.subject.meshSmoke
dc.subject.meshGene Expression Regulation, Plant
dc.subject.meshWine
dc.titleTranscription Profiling of Glycosyltransferases in Vitis vinifera Cultivars Following Smoke Exposure
dc.typeJournal article
pubs.publication-statusPublished online

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