Activation of nuclear transfer embryos
Date
2002
Authors
Grupen, C.
Nottle, M.
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Patent
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Abstract
Methods for the activation of nuclear transferred embryos using elevated calcium levels introduced into cells, and a maturation promoting factor (MPF) inhibitor are described. Elevated calcium levels introduced into the embryo cells from a culture medium containing elevated calcium levels, in the range 2 mM to about 12 mM, followed directly and immediately by incubation with DMAP are described. Also described are animals produced from embryos so treated.
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Description
European Patent EP1358317 CA Patent Number: CA2434394