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Particle coating alters mucociliary transit in excised rat trachea: A synchrotron X-ray imaging study

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dc.contributor.authorGardner, M.T.
dc.contributor.authorMcCarron, A.
dc.contributor.authorMorgan, K.
dc.contributor.authorParsons, D.
dc.contributor.authorDonnelley, M.
dc.date.issued2019
dc.description.abstractWe have previously developed non-invasive in vivo mucociliary transport (MCT) monitoring methods using synchrotron phase contrast X-ray imaging (PCXI) to evaluate potential therapies for cystic fibrosis (CF). However, previous in vivo measurements of MCT velocity using this method were lower than those from alternate methods. We hypothesise this was due to the surface chemistry of the uncoated particles. We investigated the effect of particle surface coating on MCT marker performance by measuring the velocity of uncoated, positively-charged (aminated; NH2), and negatively-charged (carboxylated; COOH) particles. The effect of aerosolised hypertonic saline (HS) was also investigated, as previous in vivo measurements showed HS significantly increased MCT rate. PCXI experiments were performed using an ex vivo rat tracheal imaging setup. Prior to aerosol delivery there was little movement of the uncoated particles, whilst the NH2 and COOH particles moved with MCT rates similar to those previously reported. After application of HS the uncoated and COOH particle velocity increased and NH2 decreased. This experiment validated the use of COOH particles as MCT marker particles over the uncoated and NH2 coated particles. Our results suggest that future experiments measuring MCT using synchrotron PCXI should use COOH coated marker particles for more accurate MCT quantification.
dc.description.statementofresponsibilityMark Gardner, Alexandra McCarron, Kaye Morgan, David Parsons, Martin Donnelley
dc.identifier.citationScientific Reports, 2019; 9(1):10983-1-10983-10
dc.identifier.doi10.1038/s41598-019-47465-1
dc.identifier.issn2045-2322
dc.identifier.issn2045-2322
dc.identifier.orcidGardner, M.T. [0000-0003-4340-2547]
dc.identifier.orcidMcCarron, A. [0000-0002-6045-3998]
dc.identifier.orcidParsons, D. [0000-0002-8775-3501] [0000-0003-1746-3290]
dc.identifier.orcidDonnelley, M. [0000-0002-5320-7756]
dc.identifier.urihttp://hdl.handle.net/2440/123889
dc.language.isoen
dc.publisherNature Publishing Group
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/GNT1079712
dc.relation.granthttp://purl.org/au-research/grants/arc/FT180100374
dc.rights© The Author(s) 2019. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.source.urihttps://doi.org/10.1038/s41598-019-47465-1
dc.subjectTrachea
dc.subjectAnimals
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectRadiography
dc.subjectMucociliary Clearance
dc.subjectEquipment Design
dc.subjectParticle Size
dc.subjectSurface Properties
dc.subjectSynchrotrons
dc.subjectX-Rays
dc.subjectFemale
dc.titleParticle coating alters mucociliary transit in excised rat trachea: A synchrotron X-ray imaging study
dc.typeJournal article
pubs.publication-statusPublished

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