Cytokine production by human milk cells and peripheral blood mononuclear cells from the same mothers
Date
2002
Authors
Hawkes, J.
Bryan, D.
Gibson, R.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Journal of Clinical Immunology, 2002; 22(6):338-344
Statement of Responsibility
Joanna S. Hawkes, Dani-Louise Bryan and Robert A. Gibson
Conference Name
Abstract
Samples of milk (n=80) and venous blood were collected at 5 weeks postpartum from 82 lactating mothers. Human milk cells and peripheral blood mononuclear cells were isolated and the production of interleukin-1β, interleukin-6, and tumor necrosis factor-α in the absence and presence of lipopolysaccharide was determined by enzyme-linked immunosorbent assay. Human milk cells spontaneously produced significantly less interleukin-1β, interleukin-6, and tumor necrosis factor-α than peripheral blood mononuclear cells in the absence of stimulation. In vitro stimulation of human milk cells with lipopolysaccharide (500 ng/ml) for 24 hr increased cytokine production by approximately 40–50%, whereas peripheral blood mononuclear cells responded to lipopolysaccharide (200 ng/ml) with increased cytokine production of up to 350%. These observations suggest that cells in milk are capable of active involvement in the production of the interleukin-1β, interleukin-6, and tumor necrosis factor-α in the mammary gland and have the capacity to respond to further stimulation after leaving the breast.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
© Springer, Part of Springer Science+Business Media