Erosion-inhibitino Fluoride-enricheE p_otential of new Stannous CPP-ACP complex
Date
2025
Authors
Al Saady, Deena Talib
Editors
Advisors
Richards, Lindsay
Ranjitkar, Sarbin
Ranjitkar, Sarbin
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Thesis
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Abstract
Erosive tooth wear has emerged as a growing concern globally, emphasising the imperative to advance our comprehension of its aetiology, prevention and management. The clinical management of erosive tooth wear should prioritise early detection and prevention over an immediate inclination toward a restorative approach. Stannous fluoride (SnF₂) and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) used individually have demonstrated anticariogenic efficacy, but its anti-erosive potential in combination is yet to be assessed. The aim of this thesis was to assess the anti-erosive properties of the synergistic impact of the SnF₂/CPPACP complex by examining the physical and chemical alterations on the enamel surface under an acidic condition (pH 3.0). Two in vitro methods of erosive enamel wear were used: the first without mechanical wear and the second involving nano-scratch testing of eroded enamel under a load of 100 mN in a nanoindenter. Then, 3D laser confocal microscopy was used to analyse erosive enamel wear both quantitatively and qualitatively. Additionally, the chemical composition of the enamel surfaces was assessed by using X-ray Photoelectron Spectroscopy (XPS) and Scanning Electron Microscopy (SEM). SnF₂ and CPP-ACP demonstrated individual capabilities as well as synergistic effect in protecting against erosive wear. This synergistic effect provided by the linking of Sn to the peptide bond of CPP-ACP can be attributed to several mechanisms, including the deposition of calcium (Ca), tin (Sn), and calcium fluoride (CaF₂) on the enamel surface to prevent erosive wear. Furthermore, the inherent ingredients of the SnF₂/CPP-ACP paste, including glycerol, silica, and guar gum, could also have lubricated the wear interface and reduced erosive wear under mechanical loading. In conclusion, the data offer novel insights into the roles of SnF₂/CPP-ACP in inhibiting erosive enamel wear, providing a foundation for future experimental and clinical studies on wear prevention.
School/Discipline
Adelaide Dental School
Dissertation Note
Thesis (Ph.D.) -- University of Adelaide, Adelaide Dental School, 2024
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