Improved detection of helix-turn-helix DNA-binding motifs in protein sequences
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1990
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Dodd, I.B.
Egan, J.B.
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Nucleic Acids Research (NAR), 1990; 18(17):5019-5026
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Ian B.Dodd and J.Barry Egan
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We present an update of our method for systematic detection and evaluation of potential hellx-turn-helix DNA-binding motifs in protein sequences [Dodd, I. and Egan, J. B. (1987) J. Mol. Biol. 194, 557-564]. The new method is considerably more powerful, detecting approximately 50% more likely hellx-turn-helix sequences without an Increase In false predictions. This improvement is due almost entirely to the use of a much larger reference set of 91 presumed helix-tumhellx sequences. The scoring matrix derived from this reference set has been calibrated against a large protein sequence database so that the score obtained by a sequence can be used to give a practical estimation of the probability that the sequence Is a helix-turn-helix motif.
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© 1990 Oxford University Press