Synthesis of steroidal inhibitors for Mycobacterium tuberculosis

Date

2024

Authors

Churchman, L.R.
Beckett, J.R.
Tan, L.
Woods, K.
Doherty, D.Z.
Ghith, A.
Bernhardt, P.V.
Bell, S.G.
West, N.P.
De Voss, J.J.

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Journal article

Citation

Journal of Steroid Biochemistry and Molecular Biology, 2024; 239:106479-1-106479-16

Statement of Responsibility

Luke R. Churchman, James R. Beckett, Lendl Tan, Kyra Woods, Daniel Z. Doherty, Amna Ghith, Paul V. Bernhardt, Stephen G. Bell, Nicholas P. West, James J. De Voss

Conference Name

Abstract

Oxidised derivatives of cholesterol have been shown to inhibit the growth of Mycobacterium tuberculosis (Mtb). The bacteriostatic activity of these compounds has been attributed to their inhibition of CYP125A1 and CYP142A1, two metabolically critical cytochromes P450 that initiate degradation of the sterol side chain. Here, we synthesise and characterise an extensive library of 28 cholesterol derivatives to develop a structure-activity relationship for this class of inhibitors. The candidate compounds were evaluated for MIC with virulent Mtb and in binding studies with CYP125A1 and CYP142A1 from Mtb.

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Dissertation Note

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Available online 10 February 2024

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© 2024 Published by Elsevier Ltd.

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