Antigen-specific ANCA ELISAs have different sensitivities for active and treated vasculitis and for nonvasculitic disease
Date
2008
Authors
Trevisin, M.
Pollock, W.
Dimech, W.
Melny, J.
Paspalionis, B.
Gillis, D.
Wong, R.
Savige, J.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
American Journal of Clinical Pathology, 2008; 129(1):42-53
Statement of Responsibility
Michelle Trevisin, Wendy Pollock, Wayne Dimech, Joy Melny, Bill Paspaliaris, David Gillis, Richard Wong and Judy Savige
Conference Name
Abstract
This study evaluated the performance of 12 assays for antineutrophil cytoplasmic antibodies (ANCA) directed against proteinase 3 (PR3) and myeloperoxidase (MPO) in 55 active and 68 treated cases of vasculitis and in nonvasculitic disease. It examined within- and between-assay precision; binding curves, binding levels, and interassay consistency; and sensitivity, specificity, and receiver operating characteristic analysis. All assays were highly sensitive for active vasculitis (median, 94%; range, 91%-96%), but sensitivities were more varied in treated disease (median, 69%; range, 57%-82%). Binding curves and binding levels were also very variable in PR3-ANCA and MPO-ANCA assays. This has implications for studies correlating ANCA levels with disease activity and in developing ANCA-based treatment guidelines. PR3-ANCA and MPO-ANCA assays need to be standardized as a matter of urgency, but in the meantime, individual laboratories must understand the limitations of the assays used, especially with low-level ANCA in treated vasculitis and nonvasculitic disease.
School/Discipline
Dissertation Note
Provenance
Description
Copyright © by the American Society for Clinical Pathology