Current studentsExisting staffContact us
 

Transgenic zebrafish recapitulating tbx16 gene early developmental expression

Simple item page
dc.contributor.authorWells, S.
dc.contributor.authorNornes, S.
dc.contributor.authorLardelli, M.
dc.contributor.editorEscriva, H.
dc.date.issued2011
dc.description.abstractWe describe the creation of a transgenic zebrafish expressing GFP driven by a 7.5 kb promoter region of the tbx16 gene. This promoter segment is sufficient to recapitulate early embryonic expression of endogenous tbx16 in the presomitic mesoderm, the polster and, subsequently, in the hatching gland. Expression of GFP in the transgenic lines later in development diverges to some extent from endogenous tbx16 expression with the serendipitous result that one line expresses GFP specifically in commissural primary ascending (CoPA) interneurons of the developing spinal cord. Using this line we demonstrate that the gene mafba (valentino) is expressed in CoPA interneurons.
dc.description.statementofresponsibilitySimon Wells, Svanhild Nornes and Michael Lardelli
dc.identifier.citationPLoS One, 2011; 6(6):e21559-1-e21559-6
dc.identifier.doi10.1371/journal.pone.0021559
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.orcidLardelli, M. [0000-0002-4289-444X]
dc.identifier.urihttp://hdl.handle.net/2440/66431
dc.language.isoen
dc.publisherPublic Library of Science
dc.rightsCopyright 2011 Wells et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.source.urihttps://doi.org/10.1371/journal.pone.0021559
dc.subjectSpinal Cord
dc.subjectNeurons
dc.subjectInterneurons
dc.subjectEmbryo, Nonmammalian
dc.subjectAnimals
dc.subjectAnimals, Genetically Modified
dc.subjectZebrafish
dc.subjectT-Box Domain Proteins
dc.subjectZebrafish Proteins
dc.subjectGreen Fluorescent Proteins
dc.subjectOrgan Specificity
dc.subjectGene Expression Regulation, Developmental
dc.subjectGenome
dc.subjectFluorescence
dc.titleTransgenic zebrafish recapitulating tbx16 gene early developmental expression
dc.typeJournal article
pubs.publication-statusPublished

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
hdl_66431.pdf
Size:
507.81 KB
Format:
Adobe Portable Document Format
Description:
Published version
Download

Collections

Molecular and Biomedical Science publications