Towards cloning the self-incompatibility genes from Phalaris coerulescens
| dc.contributor.advisor | Langridge, Peter | en |
| dc.contributor.advisor | Barker, Susan Jane | en |
| dc.contributor.author | Bian, Xue-Yu | en |
| dc.contributor.school | Department of Plant Science | en |
| dc.date.issued | 2001 | en |
| dc.description.abstract | Self-incompatibility (SI) is an important genetic mechanism to prevent the inbreeding of flowering plants and also an excellent system for studying cell-cell recognition and signal transduction. During evolution, several SI systems have been evolved. A unique SI system widely spreads in the grasses. In the grasses, two unlinked, multi-allelic loci (S and Z) determine SI specificity. A putative self-incompatibility gene (Bm2) was previously cloned. In this study, the role of Bm2 in self-incompatibility was investigated first. The cDNA homologues of Bm2 were sequenced from two pollen-only mutants. The results indicated that Bm2 is not the one of SI genes in Phalaris, but represents a subclass of thioredoxin h. Thus a map-based cloning strategy was then adopted to clone the SI genes from Phalaris. Fine linkage maps of the S and Z regions were constructed. RFLP probes from wheat, barley, oat and rye were screened and the S locus was delimited to 0.26 cM and the Z locus to 1.0 cM from one side using specially designed segregating populations. The S locus was located to the sub-centromere region of triticeae chromosome group 1 and the Z locus to the middle of the long arm of group 2. Finally, barley and rice bacterial artificial chromosome (BAC) clones corresponding to the S and Z region were identified to analyse the chromosome structures and to seek candidate SI genes. The abundant repetitive sequences in the identified barley BAC clones limit their usefulness. Identification of Rice BAC clones orthologous to the S and Z regions open the gate to use rice genome information to clone SI genes from the grasses. A positive rice clone (139.9 kb) orthologous to the S region contained 19 predicted genes. Several of these genes might be involved in pollen tube germination and pollen-stigma interaction, which are the major parts of SI reaction. A positive clone (118.9 kb) orthologous to the Z region gave 16 predicted genes. The predicted genes on the outmost ends of these clones could be used to construct contigs to cover the S and Z regions and delimit the S and Z loci in the grasses. | en |
| dc.description.dissertation | Thesis (Ph.D.)--Department of Plant Science, 2001. | en |
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| dc.format.mimetype | application/pdf | en |
| dc.format.mimetype | application/pdf | en |
| dc.format.mimetype | application/pdf | en |
| dc.format.mimetype | application/pdf | en |
| dc.format.mimetype | application/pdf | en |
| dc.format.mimetype | application/pdf | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.uri | http://hdl.handle.net/2440/37906 | |
| dc.language.iso | en | en |
| dc.provenance | This electronic version is made publicly available by the University of Adelaide in accordance with its open access policy for student theses. Copyright in this thesis remains with the author. This thesis may incorporate third party material which has been used by the author pursuant to Fair Dealing exception. If you are the author of this thesis and do not wish it to be made publicly available or If you are the owner of any included third party copyright material you wish to be removed from this electronic version, please complete the take down form located at: http://www.adelaide.edu.au/legals | |
| dc.subject | Phalaris genetics, Phalaris pollen, Phalaris coerulescens, distorted segregating mapping, synteny mapping, map-based cloning | en |
| dc.title | Towards cloning the self-incompatibility genes from Phalaris coerulescens | en |
| dc.type | Thesis | en |