A novel red-emitting aggregation-induced emission probe for determination of β-glucosidase activity
Date
2023
Authors
Yao, B.
Zhao, J.
Ding, S.
Giel, M.C.
Zhang, G.
Ding, D.
Tang, Y.
Weng, Z.H.
Hong, Y.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Biomaterials, 2023; 295:122046-1-122046-11
Statement of Responsibility
Bicheng Yao, Jiamin Zhao, Siyang Ding, Marie-Claire Giel, Guoqiang Zhang, Dan Ding, Youhong Tang, Zhe H. Weng, Yuning Hong
Conference Name
Abstract
beta-Glucosidase (beta-Glu) is a ubiquitous enzyme which has multiple roles in medical diagnosis, food production, agriculture, etc. Existing beta-Glu assays have limitations such as complex operation, long running time, and high background noise. Here we report a red-emissive probe TBPG for measuring the activity of beta-Glu. The probe was synthesized through conjugating a beta-Glu targeting glucoside to an aggregation-induced emission (AIE) fluorophore. In the presence of beta-Glu, TBPG was hydrolyzed and exhibited a fluorescence turn-on process. The detection conditions including time, temperature, pH value, buffer, and probe concentration were optimized systematically. Afterwards, fluorescence titration was conducted showing an excellent linearity (R2 = 0.998), a wide linear dynamic range (0-5.0 U/mL), and a limit of detection as low as 0.6 U/L. The detection specificity and ion interference were evaluated by adding various biological species and ions to probe without or with beta-Glu. Next, we demonstrate the applicability of probe TBPG in determining the beta-Glu activity in living cells using confocal microscopy and flow cytometry. Finally, this newly established assay was applied to real soil samples. Comparable results were obtained as the commercial assay, manifesting its great potential in soil enzyme analysis.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
© 2023 Elsevier Ltd. All rights reserved.