Current studentsExisting staffContact us
 

Fluorescence-activated cell sorting for analysis of cell type-specific responses to salinity stress in Arabidopsis and rice

Simple item page
dc.contributor.authorEvrard, A.
dc.contributor.authorBargmann, B.
dc.contributor.authorBirnbaum, K.
dc.contributor.authorTester, M.
dc.contributor.authorBaumann, U.
dc.contributor.authorJohnson, A.
dc.contributor.editorShabala, S.
dc.contributor.editorCuin, T.
dc.date.issued2012
dc.description.abstractFluorescence-activated cell sorting (FACS) provides a rapid means of isolating large numbers of fluorescently tagged cells from a heterogeneous mixture of cells. Collections of transgenic plants with cell type-specific expression of fluorescent marker genes such as green fluorescent protein (GFP) are ideally suited for FACS-assisted studies of individual cell types. Here we describe the use of Arabidopsis and rice enhancer trap lines with tissue-specific GFP expression patterns in the root to isolate specific cell types of root tissues using FACS. Additionally, protocols are provided to impose a ramped salinity stress for 48 h prior to cell sorting.
dc.description.statementofresponsibilityAurelie Evrard, Bastiaan O. R. Bargmann, Kenneth D. Birnbaum, Mark Tester, Ute Baumann, and Alexander A. T. Johnson
dc.identifier.citationPlant Salt Tolerance: Methods and Protocols, 2012 / Shabala, S., Cuin, T. (ed./s), vol.913, pp.265-276
dc.identifier.doi10.1007/978-1-61779-986-0_18
dc.identifier.isbn9781617799853
dc.identifier.orcidBaumann, U. [0000-0003-1281-598X]
dc.identifier.urihttp://hdl.handle.net/2440/77889
dc.language.isoen
dc.publisherHumana Press
dc.publisher.placeUSA
dc.relation.ispartofseriesMethods in Molecular Biology; 913
dc.rights© Springer Science+Business Media, LLC 2012
dc.source.urihttps://doi.org/10.1007/978-1-61779-986-0_18
dc.subjectProtoplasts
dc.subjectPlants, Genetically Modified
dc.subjectArabidopsis
dc.subjectPlant Roots
dc.subjectFlow Cytometry
dc.subjectCell Separation
dc.subjectGermination
dc.subjectSalinity
dc.subjectStress, Physiological
dc.subjectPlant Cells
dc.subjectOryza
dc.subjectSalt Tolerance
dc.titleFluorescence-activated cell sorting for analysis of cell type-specific responses to salinity stress in Arabidopsis and rice
dc.typeBook chapter
pubs.publication-statusPublished

Files

Collections

Agriculture, Food and Wine publications