Eomesodermin controls interleukin-5 production in memory T helper 2 cells through inhibition of activity of the transcription factor GATA3
Date
2011
Authors
Endo, Y.
Iwamura, C.
Kuwahara, M.
Suzuki, A.
Sugaya, K.
Tumes, D.J.
Tokoyoda, K.
Hosokawa, H.
Yamashita, M.
Nakayama, T.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Immunity, 2011; 35(5):733-745
Statement of Responsibility
Conference Name
Abstract
The regulation of memory CD4 + helper T (Th) cell function, such as polarized cytokine production, remains unclear. Here we show that memory T helper 2 (Th2) cells are divided into four subpopulations by CD62L and CXCR3 expression. All four subpopulations produced interleukin-4 (IL-4) and IL-13, whereas only the CD62L lo CXCR3 lo population produced IL-5 accompanied by increased H3-K4 methylation at the Il5 gene locus. The transcription factor Eomesodermin (encoded by Eomes) was highly expressed in memory Th2 cells, whereas its expression was selectively downregulated in the IL-5-producing cells. Il5 expression was enhanced in Eomes-deficient cells, and Eomesodermin was shown to interact with the transcription factor GATA3, preventing GATA3 binding to the Il5 promoter. Memory Th2 cell-dependent airway inflammation was attenuated in the absence of the CD62L lo CXCR3 lo population but was enhanced by Eomes-deficient memory Th2 cells. Thus, IL-5 production in memory Th2 cells is regulated by Eomesodermin via the inhibition of GATA3 activity.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
Copyright 2011 Elsevier