Promoter region methylation does not account for the frequent loss of expression of the Fas gene in colorectal carcinoma
Date
2000
Authors
Butler, L.
Dobrovic, A.
Bianco-Miotto, T.
Cowled, P.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
British Journal of Cancer, 2000; 82(1):131-135
Statement of Responsibility
LM Butler, A Dobrovic, T Bianco and PA Cowled
Conference Name
Abstract
Expression of the apoptosis-promoting Fas gene is frequently reduced or lost during the development of colorectal carcinoma. However, loss of heterozygosity at the Fas locus or Fas gene rearrangements do not account for the loss of expression of Fas, raising the possibility that methylation of the Fas promoter may inhibit gene expression in colorectal carcinomas. We have examined the Fas promoter region CpG island for evidence of hypermethylation in colorectal tumours. Forty-seven specimens of colorectal adenoma and carcinoma, as well as six samples of normal colonic mucosa, were examined by Southern blotting for methylation at HpaII and Cfol sites in this region. No methylation was detected in any of the specimens, suggesting that hypermethylation is not primarily responsible for the loss of expression of the Fas gene during colorectal tumorigenesis.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
© 2000 Cancer Research Campaign