Behavior of lysozyme at the electrified water/room temperature ionic liquid interface
Date
2012
Authors
Alvarez de Eulate, E.
Silvester, D.S.
Arrigan, D.W.M.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Chemistry - An Asian Journal, 2012; 7(11):2559-2561
Statement of Responsibility
Conference Name
Abstract
The electrochemical behavior of bioactive molecules at the interface between two immiscible electrolyte solutions (ITIES) has been studied recently to better understand biological processes, biopharmaceutical stability, and bioanalytical measurements. Importantly, behavior at the ITIES can provide the foundations for new label-free and sensitive biomolecular detection tools. To date, drugs, neurotransmitters, carbohydrates, proteins, peptides, amino acids, and DNA have been studied at the ITIES.
Herein, we report on the behavior of a model protein, hen egg white lysozyme (HEWL), at the W|RTIL interface, building on our previous study on the formation of a W|RTIL microinterface array and on the behavior of proteins at electrified water|organic solvent interfaces. By using an experimental set-up based on a microinterface array formed at the pores of a micromachined silicon membrane, the behavior of HEWL was examined using voltammetric methods. The ionic liquid employed in all of these studies was trihexyl(tetradecyl)phosphonium tris(pentafluoro-ethyl)trifluorophosphate , in conjunction with a water phase containing either lithium chloride or hydrochloric acid.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
Copyright 2012 Wiley VCH