The separation of arsenic species in soils and plant tissues by anion-exchange chromatography with inductively coupled mass spectrometry using various mobile phases
Date
2008
Authors
Chen, Z.
Akter, K.F.
Rahman, M.M.
Naidu, R.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Microchemical Journal, 2008; 89(1):20-28
Statement of Responsibility
Conference Name
Abstract
Anion-exchange chromatography (Hamilton, PRP-X100) with inductively coupled plasma mass spectrometry (ICP-MS) is commonly used for the speciation of arsenic in environmental and biological samples. However, retentions for As species are frequently different because of the use of widely different mobile phases. In addition, chloride in matrices interferes with arsenic determination. In this study, we systematically investigated various mobile phases based on ammonium salts affecting arsenic retention to eliminate chloride interference chromatographically. Hence, various mobile phases based on ammonium salts, including NH<inf>4</inf>H<inf>2</inf>PO<inf>4</inf>, NH<inf>4</inf>HPO<inf>4</inf>, NH<inf>4</inf>Ac, NH<inf>4</inf>HCO<inf>3</inf> and NH<inf>4</inf>NO<inf>3</inf>, were examined for reasonable resolution and to separate chloride from arsenic species. The best result was obtained with a mobile phase containing 30 mM NH<inf>4</inf>H<inf>2</inf>PO<inf>4</inf> at pH 5.6, where the separation of arsenic species, including arsenite [As(III)], arsenate [As(V)], dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA)], was achieved within 9 minutes with reasonable resolution and free of chloride interference at its high level (500 mg L<sup>- 1</sup>). The detection limits for the arsenic species were in the range of 0.1-0.3 μg L<sup>- 1</sup> with a direct injection of sample without removing matrix. Finally, the proposed method was used for the determination of arsenic species in contaminated soil and plant tissues. © 2007 Elsevier B.V. All rights reserved.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
Copyright status unknown