Single-base-resolution and long-coverage sequencing based on single-molecule nanomanipulation
Date
2007
Authors
An, H.
Huang, J.
Lu, M.
Li, X.
Lu, J.
Li, H.
Zhang, Y.
Li, M.
Hu, J.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Nanotechnology, 2007; 18(22):225101-
Statement of Responsibility
Hongjie An,Jiehuan Huang, Ming Lü, Xueling Li, Junhong Lü, Haikuo Li, Yi Zhang, Minqian Li and Jun Hu
Conference Name
Abstract
We show new approaches towards a novel single-molecule sequencing strategy which consists of high-resolution positioning isolation of overlapping DNA fragments with atomic force microscopy (AFM), subsequent single-molecule PCR amplification and conventional Sanger sequencing. In this study, a DNA labelling technique was used to guarantee the accuracy in positioning the target DNA. Single-molecule multiplex PCR was carried out to test the contamination. The results showed that the two overlapping DNA fragments isolated by AFM could be successfully sequenced with high quality and perfect contiguity, indicating that single-base resolution and long-coverage sequencing have been achieved simultaneously.
School/Discipline
Dissertation Note
Provenance
Description
© 2007 IOP Publishing Ltd