Understanding polysulfide evolution in wine: insights from accelerated ageing and real-time cellaring in different packaging

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2026

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Hou, Y.
Bekker, M.Z.
Siebert, T.E.
Kreitman, G.Y.
Jeffery, D.W.

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Food Chemistry: X, 2026; 33:103447-1-103447-14

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Yu Hou, Marlize Z. Bekker, Tracey E. Siebert, Gal Y. Kreitman, David W. Jeffery

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Abstract

Volatile sulfur compounds (VSCs) and non-volatile biothiols play a crucial role in wine quality. Certain sulfhydryl compounds can react to form odourless polysulfides (RSSnSR′) that potentially contribute to the development of sulfur off-odours during winemaking and storage. This study investigated the evolution of sulfur species in Chardonnay and Shiraz wine during real-time and accelerated ageing in glass bottles with different closures and in aluminium cans. Moderate glutathione (GSH) accumulation was accompanied by disulfide formation, with GSH trisulfide (GS-S-SG), tetrasulfide (GS-S2-SG), and mixed cysteine-GSH tetrasulfide (Cys-S2-SG) displaying compound- and matrix-specific trends. Can packaging maintained higher amounts of VSCs, sulfur dioxide, and GSH, whereas closures with varying oxygen transmission had the most pronounced impact on glutathione disulfide (GS-SG) accumulation. Treatment with nitrogen gas or copper exhibited preservation effects under accelerated ageing. These findings highlight the role of wine packaging on polysulfide chemistry and clarify the effects of pre-bottling strategies for VSC management.

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© 2025 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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