Effect of vaccine diluents on the colonization of Salmonella Typhimurium vaccine in chickens

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dc.contributor.authorJia, S.
dc.contributor.authorMcWhorter, A.R.
dc.contributor.authorKhan, S.
dc.contributor.authorWillson, N.L.
dc.contributor.authorAndrews, D.M.
dc.contributor.authorUnderwood, G.J.
dc.contributor.authorChousalkar, K.K.
dc.date.issued2024
dc.description.abstractOne of the commonly used methods to control on-farm Salmonella outbreaks in the poultry industry is vaccination. Vaccine diluents may affect vaccine efficacy but there are limited studies on how different diluents affect the colonization of live, attenuated vaccines. In this study, buffered peptone water, Marek’s diluent and water were used as the diluents to reconstitute the commercially available Salmonella Typhimurium vaccine (Vaxsafe® ST). The diluent reconstituted vaccine was then given to day-old layer hens to test its effects on gut colonization. The vaccine reconstituted in Marek’s diluent and buffered peptone water had a significantly higher colonization rate at 7 days post-vaccination in liver, spleen, oesophagus, crop, and proventriculus. At 14 days postvaccination, there was a significantly higher vaccine load in the upper digestive tract of chickens that received vaccine reconstituted in Marek’s diluent and buffered peptone water. The Marek’s diluent and buffered peptone water reconstituted vaccine had a higher detection rate in the chicken’s liver and spleen. Colonisation with water as a diluent was higher than the others especially in the lower parts of the intestine at 7 days postvaccination. There was non-significant variation in the antibody titers at 7- and 14-days post-vaccination. In general, the results demonstrated that the use of nutrient-rich diluents in reconstituting live, attenuated Salmonella vaccine can enhance vaccine colonization. This study establishes a foundation for future work aimed at optimising strategies for vaccine reconstitution, crucial for eliciting a robust immune response in the host.
dc.description.statementofresponsibilitySiyuan Jia, Andrea R. McWhorter, Samiullah Khan, Nicky-Lee Willson, Daniel M. Andrews, Gregory J. Underwood, Kapil K. Chousalkar
dc.identifier.citationVeterinary and Animal Science, 2024; 26:100409-1-100409-8
dc.identifier.doi10.1016/j.vas.2024.100409
dc.identifier.issn2451-943X
dc.identifier.issn2451-943X
dc.identifier.orcidJia, S. [0000-0002-8483-738X]
dc.identifier.orcidMcWhorter, A.R. [0000-0002-5793-1629]
dc.identifier.orcidKhan, S. [0000-0002-0292-2979]
dc.identifier.orcidWillson, N.L. [0000-0002-3851-8548]
dc.identifier.orcidChousalkar, K.K. [0000-0002-4134-3996]
dc.identifier.urihttps://hdl.handle.net/2440/144226
dc.language.isoen
dc.publisherElsevier
dc.relation.granthttp://purl.org/au-research/grants/arc/LP190100864
dc.rights© 2024 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
dc.source.urihttp://dx.doi.org/10.1016/j.vas.2024.100409
dc.subjectSalmonella vaccination; Live bacterial vaccine; Vaccine diluent
dc.titleEffect of vaccine diluents on the colonization of Salmonella Typhimurium vaccine in chickens
dc.typeJournal article
pubs.publication-statusPublished

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