The degradation of curcuminoids in a human faecal fermentation model

Date

2015

Authors

Tan, S.
Calani, L.
Bresciani, L.
Dall'asta, M.
Faccini, A.
Augustin, M.A.
Gras, S.L.
Del Rio, D.

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Journal article

Citation

International Journal of Food Sciences and Nutrition, 2015; 66(7):790-796

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Suryani Tan, Luca Calani, Letizia Bresciani, Margherita Dall, asta, Andrea Faccini, Mary Ann Augustin, Sally L. Gras, Daniele Del Rio

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Abstract

In the present study, the colonic metabolism of three curcuminoids (80.1% curcumin, 15.6%, demethoxycurcumin (DMC) and 2.6% bis-demethoxycurcumin (Bis-DMC)) was evaluated using an in vitro model containing human faecal starters. The breakdown products formed were identified and characterized using different analytical platforms. Following in vitro incubation, the relative amounts of degraded curcuminoids and the produced metabolites were analyzed using a UHPLC coupled with a linear ion trap mass spectrometer, with the addition of hybrid ion trap-Orbitrap Mass Spectrometer when required. Up to 24% of curcumin, 61% of demethoxycurcumin (DMC) and 87% of bis-demethoxycurcumin (Bis-DMC) were degraded by the human faecal microbiota after 24 h of fermentation in vitro. Three main metabolites, namely tetrahydrocurcumin (THC), dihydroferulic acid (DFA) and a metabolite with an accurate mass of 181.08734, which was tentatively identified as 1-(4-Hydroxy-3-methoxyphenyl)-2-propanol were detected in the fermentation cultures containing the curcuminoids. The data presented here provide insights into curcuminoid colonic metabolism, showing that bacterial breakdown products should be considered in further studies on both bioavailability bioactivity of curcumin.

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© 2015 Taylor & Francis.

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