Rapid detection of clenbuterol in milk using microfluidic paper-based ELISA
Date
2018
Authors
Ma, L.
Nilghaz, A.
Choi, J.R.
Liu, X.
Lu, X.
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Journal article
Citation
Food Chemistry, 2018; 246:437-441
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Abstract
In this study, a paper-based microfluidic enzyme-linked immunosorbent assay (ELISA) was developed as a screening system for rapid detection of clenbuterol, which is illegally used as a growth promoter for food-producing animals. The microfluidic paper-based analytical device (μPAD) was combined with ELISA and the intrinsic properties of paper allowed the entrapment of antibody through cellulosic fibres, validating to be an alternative to 96-well ELISA microplate for food safety monitoring. Detection of clenbuterol in milk was achieved by measuring the intensity of colour change that was proportional to the analyte concentration with a detection limit of 0.2 ppb. The μPAD effectively reduces the cost, volume of reagents, and time required to run ELISA for food sample testing.
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Data source: Supplementary data, https://doi.org/10.1016/j.foodchem.2017.12.022
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Copyright 2017 Elsevier