Fish gill damage by harmful microalgae newly explored by microelectrode ion flux estimation techniques

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dc.contributor.authorMardones, J.I.
dc.contributor.authorShabala, L.
dc.contributor.authorShabala, S.
dc.contributor.authorDorantes-Aranda, J.J.
dc.contributor.authorSeger, A.
dc.contributor.authorHallegraeff, G.M.
dc.date.issued2018
dc.descriptionAvailable online 28 September 2018
dc.description.abstractHarmful algal blooms (HAB) are responsible for massive mortalities of wild and aquacultured fish due to noticeable gill damage, but the precise fish-killing mechanisms remain poorly understood. A non-invasive microelectrode ion flux estimation (MIFE) technique was successfully applied to assess changes in membrane-transport processes in a model fish gill cell line exposed to harmful microplankton. Net Ca2+, H+, K+ ion fluxes in the rainbow trout cell line RTgill-W1 were monitored before and after addition of lysed cells of this Paralytic Shellfish Toxins (PST) producer along with purified endocellular dinoflagellate PST. It was demonstrated that PST alone do not play a role in fish gill damage during A. catenella outbreaks as previously thought, but that other ichthyotoxic metabolites from lysed algal cells (i.e. lipid peroxidation products or other unknown metabolites) result in net K+ efflux from fish gill cells and thereby gill cell death.
dc.description.statementofresponsibilityJorge I. Mardones, Lana Shabala, Sergey Shabala, Juan José Dorantes-Aranda, Andreas Seger, Gustaaf M. Hallegraeff
dc.identifier.citationHarmful Algae, 2018; 80:55-63
dc.identifier.doi10.1016/j.hal.2018.09.004
dc.identifier.issn1568-9883
dc.identifier.issn1878-1470
dc.identifier.orcidSeger, A. [0000-0001-7018-0455]
dc.identifier.urihttps://hdl.handle.net/2440/138882
dc.language.isoen
dc.publisherElsevier BV
dc.relation.granthttp://purl.org/au-research/grants/arc/DP130102859
dc.rights© 2018 Elsevier B.V. All rights reserved.
dc.source.urihttps://doi.org/10.1016/j.hal.2018.09.004
dc.subjectFish-killing; Alexandrium catenella; Ichthyotoxins; PST; RTgill-W1; Cell membrane ion fluxes
dc.subject.meshCell Line
dc.subject.meshGills
dc.subject.meshAnimals
dc.subject.meshOncorhynchus mykiss
dc.subject.meshMarine Toxins
dc.subject.meshMicroelectrodes
dc.subject.meshIon Transport
dc.subject.meshHarmful Algal Bloom
dc.subject.meshMicroalgae
dc.titleFish gill damage by harmful microalgae newly explored by microelectrode ion flux estimation techniques
dc.typeJournal article
pubs.publication-statusPublished

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