Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A
Date
2012
Authors
Yang, C.
Lates, V.
Prieto SimoĢn, B.
Marty, J.L.
Yang, X.
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Journal article
Citation
Biosensors and Bioelectronics, 2012; 32(1):208-212
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Abstract
We report an aptasensor for biosensing of Ochratoxin A (OTA) using aptamer-DNAzyme hairpin as biorecognition element. The structure of this engineered nucleic acid includes the horseradish peroxidase (HRP)-mimicking DNAzyme and the OTA specific aptamer sequences. A blocking tail captures a part of these sequences in the stem region of the hairpin. In the presence of OTA, the hairpin is opened due to the formation of the aptamer-analyte complex. As a result, self-assembly of the active HRP-mimicking DNAzyme occurs. The activity of this DNAzyme is linearly correlated with OTA concentration up to 10. nM, showing a limit of detection of 2.5. nM.
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Data source: Supplementary data, http://www.sciencedirect.com/science/article/pii/S0956566311008062#appd002
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Copyright 2011 Elsevier B.V.