LRGUK-1 is required for basal body and manchette function during spermatogenesis and male fertility
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(Published version)
Date
2015
Authors
Liu, Y.
DeBoer, K.
de Kretser, D.
O Donnell, L.
O Connor, A.
Merriner, D.
Okuda, H.
Whittle, B.
Jans, D.
Efthymiadis, A.
Editors
Yan, W.
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Journal article
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PLoS Genetics, 2015; 11(3):1-20
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Yan Liu, Kathleen DeBoer, David M. de Kretser, Liza O, Donnell, Anne E. O, Connor, D. Jo Merriner, Hidenobu Okuda, Belinda Whittle, David A. Jans, Athina Efthymiadis, Robert I. McLachlan, Christopher J. Ormandy, Chris C. Goodnow, Duangporn Jamsai, Moira K. O, Bryan
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Abstract
Male infertility affects at least 5% of reproductive age males. The most common pathology is a complex presentation of decreased sperm output and abnormal sperm shape and motility referred to as oligoasthenoteratospermia (OAT). For the majority of OAT men a precise diagnosis cannot be provided. Here we demonstrate that leucine-rich repeats and guanylate kinase-domain containing isoform 1 (LRGUK-1) is required for multiple aspects of sperm assembly, including acrosome attachment, sperm head shaping and the initiation of the axoneme growth to form the core of the sperm tail. Specifically, LRGUK-1 is required for basal body attachment to the plasma membrane, the appropriate formation of the sub-distal appendages, the extension of axoneme microtubules and for microtubule movement and organisation within the manchette. Manchette dysfunction leads to abnormal sperm head shaping. Several of these functions may be achieved in association with the LRGUK-1 binding partner HOOK2. Collectively, these data establish LRGUK-1 as a major determinant of microtubule structure within the male germ line.
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© 2015 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited