Assessment of visual function in blind mice and monkeys with subretinally implanted nanowire arrays as artificial photoreceptors

Date

2023

Authors

Yang, R.
Zhao, P.
Wang, L.
Feng, C.
Peng, C.
Wang, Z.
Zhang, Y.
Shen, M.
Shi, K.
Weng, S.

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Nature Biomedical Engineering, 2023; 8(8):1-37

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Ruyi Yang ... Chen Peng ... et al.

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Abstract

Retinal prostheses could restore image-forming vision in conditions of photoreceptor degeneration. However, contrast sensitivity and visual acuity are often insufcient. Here we report the performance, in mice and monkeys with induced photoreceptor degeneration, of subretinally implanted gold-nanoparticle-coated titania nanowire arrays providing a spatial resolution of 77.5 μm and a temporal resolution of 3.92 Hz in ex vivo retinas (as determined by patch-clamp recording of retinal ganglion cells). In blind mice, the arrays allowed for the detection of drifting gratings and fashing objects at light-intensity thresholds of 15.70–18.09 μW mm–2, and ofered visual acuities of 0.3–0.4 cycles per degree, as determined by recordings of visually evoked potentials and optomotor-response tests. In monkeys, the arrays were stable for 54 weeks, allowed for the detection of a 10-μW mm–2 beam of light (0.5° in beam angle) in visually guided saccade experiments, and induced plastic changes in the primary visual cortex, as indicated by long-term in vivo calcium imaging. Nanomaterials as artifcial photoreceptors may ameliorate visual defcits in patients with photoreceptor degeneration.

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© 2023, The Author(s), under exclusive licence to Springer Nature Limited

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