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Eukaryotic elongation factor 2 kinase regulates the synthesis of microtubule-related proteins in neurons

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dc.contributor.authorKenney, J.
dc.contributor.authorGenheden, M.
dc.contributor.authorMoon, K.
dc.contributor.authorWang, X.
dc.contributor.authorFoster, L.
dc.contributor.authorProud, C.
dc.date.issued2016
dc.description.abstractModulation of the elongation phase of protein synthesis is important for numerous physiological processes in both neurons and other cell types. Elongation is primarily regulated via eukaryotic elongation factor 2 kinase (eEF2K). However, the consequence of altering eEF2K activity on the synthesis of specific proteins is largely unknown. Using both pharmacological and genetic manipulations of eEF2K combined with two protein-labeling techniques, stable isotope labeling of amino acids in cell culture and bio-orthogonal non-canonical amino acid tagging, we identified a subset of proteins whose synthesis is sensitive to inhibition of eEF2K in murine primary cortical neurons. Gene ontology (GO) analyses indicated that processes related to microtubules are particularly sensitive to eEF2K inhibition. Our findings suggest that eEF2K likely contributes to neuronal function by regulating the synthesis of microtubule-related proteins. Modulation of the elongation phase of protein synthesis is important for numerous physiological processes in neurons. Here, using labeling of new proteins coupled with proteomic techniques in primary cortical neurons, we find that the synthesis of microtubule-related proteins is up-regulated by inhibition of elongation. This suggests that translation elongation is a key regulator of cytoskeletal dynamics in neurons.
dc.description.statementofresponsibilityJustin W. Kenney, Maja Genheden, Kyung‐Mee Moon, Xuemin Wang, Leonard J. Foster, Christopher G. Proud
dc.identifier.citationJournal of Neurochemistry, 2016; 136(2):276-284
dc.identifier.doi10.1111/jnc.13407
dc.identifier.issn0022-3042
dc.identifier.issn1471-4159
dc.identifier.orcidProud, C. [0000-0003-0704-6442]
dc.identifier.urihttp://hdl.handle.net/2440/113633
dc.language.isoen
dc.publisherWiley
dc.rights© 2015 The Authors. Journal of Neurochemistry published by John Wiley & Sons Ltd on behalf of International Society for Neurochemistry. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
dc.source.urihttps://doi.org/10.1111/jnc.13407
dc.subjectElongation; mass spectrometry; microtubules; primary neurons; SILAC; translational control
dc.titleEukaryotic elongation factor 2 kinase regulates the synthesis of microtubule-related proteins in neurons
dc.typeJournal article
pubs.publication-statusPublished

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