Intracellular trafficking pathway of layered double hydroxide nanoparticles in human cells: size-dependent cellular delivery
Date
2012
Authors
Chung, H.E.
Park, D.H.
Choy, J.H.
Choi, S.J.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Applied Clay Science, 2012; 65-66:24-30
Statement of Responsibility
Conference Name
Abstract
Layered double hydroxide (LDH) nanoparticles are promising candidates as delivery carriers, since they have low toxicity and can intercalate anionic drugs or genes in the interlayer spaces of two dimensional lattices. However, little information is available about their intracellular fate and trafficking pathway in human cells, which is important to improve delivery efficiency and predict toxicity potential. From the immunofluorescence and confocal microscopic studies to evaluate intracellular colocalization of fluorescein isothiocyanate-labeled nanoparticles (50 and 100. nm) with specific compartments, we found that the cellular uptake reached a maximum level at 0.5. h and gradually decreased over time.
The present nanoparticles were highly colocalized with early endosomes within the first 0.5. h and largely found in an exocytic organelle, the Golgi apparatus over a period of 1-24. h. Most of 100. nm could escape a typical endo-lysosomal degradation, while 50. nm followed an endosome-lysosome pathway as well as exocytosis one with the same degree. A model for the intracellular trafficking of LDH nanoparticles depending on particle size is proposed on the basis of quantitative analysis data.
School/Discipline
Dissertation Note
Provenance
Description
Data source: Supplementary data, https://doi.org/10.1016/j.clay.2012.06.007
Access Status
Rights
Copyright 2015 Elsevier B.V.