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A close correlation in the expression patterns of Af-6 and Usp9x in Sertoli and granulosa cells of mouse testis and ovary

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dc.contributor.authorSato, Takeshien
dc.contributor.authorKanai, Yoshiakiraen
dc.contributor.authorNoma, Takashien
dc.contributor.authorKanai-Azuma, Masamien
dc.contributor.authorTaya, Shinichiroen
dc.contributor.authorMatsui, Toshiyasuen
dc.contributor.authorIshii, Makien
dc.contributor.authorKawakami, Hayatoen
dc.contributor.authorKurohmaru, Masamichien
dc.contributor.authorKaibuchi, Kozoen
dc.contributor.authorWood, Stephen Andrewen
dc.contributor.authorHayashi, Yoshihiroen
dc.contributor.organisationCentre for the Molecular Genetics of Developmenten
dc.date.issued2004en
dc.descriptionCopyright © 2004 by the Society for Reproduction and Fertilityen
dc.description.abstractUsp9x, an X-linked deubiquitylating enzyme, is stage dependently expressed in the supporting cells (i.e. Sertoli cells and granulosa cells) and germ cells during mouse gametogenesis. Af-6, a cell junction protein, has been identified as a substrate of Usp9x, suggesting a possible association between Usp9x and Af-6 in spermatogenesis and oogenesis. In this study, we examined the expression pattern of Af-6 and Usp9x and their intracellular localization in testes and ovaries of mice treated with or without pregnant mare serum gonadotropin (PMSG), an FSH-like hormone. In both testes and ovaries, Af-6 expression was predominantly observed in supporting cells, as well as in steroidogenic cells, but not in any germ cells. In Sertoli cells, Af-6 was continuously expressed throughout postnatal and adult stages, where both Af-6 and Usp9x were enriched at the sites of Sertoli–Sertoli and Sertoli–spermatid junctions especially at stages XI–VI. In the granulosa cells, Af-6, as well as Usp9x, was highly expressed in primordial and primary follicles, but its expression rapidly decreased after the late-secondary follicle stage. Interestingly, in PMSG-treated mice, the expression levels of Af-6 and Usp9x were synchronously enhanced, slightly in Sertoli cells and strongly in granulosa cells of the late-secondary and Graafian follicles. Such closely correlated expression patterns between Af-6 and Usp9x clearly suggest that Af-6 may be deubiquitylated by Usp9x in both Sertoli and granulosa cells. It further suggests that the post-translational regulation of Af-6 by Usp9x may be one potential pathway to control the cell adhesion dynamics in mammalian gametogenesis.en
dc.description.statementofresponsibilityTakeshi Sato, Yoshiakira Kanai, Takashi Noma, Masami Kanai-Azuma, Shinichiro Taya, Toshiyasu Matsui, Maki Ishii, Hayato Kawakami, Masamichi Kurohmaru, Kozo Kaibuchi, Stephen A Wood and Yoshihiro Hayashien
dc.identifier.citationReproduction, 2004; 128:583-594en
dc.identifier.doi10.1530/rep.1.00060en
dc.identifier.issn1470-1626en
dc.identifier.urihttp://hdl.handle.net/2440/3154
dc.language.isoenen
dc.publisherBio Scientificaen
dc.titleA close correlation in the expression patterns of Af-6 and Usp9x in Sertoli and granulosa cells of mouse testis and ovaryen
dc.typeJournal articleen

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