Atg9 is required for intraluminal vesicles in amphisomes and autolysosomes

Simple item page
dc.contributor.authorBader, C.
dc.contributor.authorShandala, T.
dc.contributor.authorNg, Y.
dc.contributor.authorJohnson, I.
dc.contributor.authorBrooks, D.
dc.date.issued2015
dc.descriptionData source: Supplementary data, http://bio.biologists.org/content/suppl/2015/09/04/bio.013979.DC1
dc.description.abstractAutophagy is an intracellular recycling and degradation process, which is important for energy metabolism, lipid metabolism, physiological stress response and organism development. During Drosophila development, autophagy is up-regulated in fat body and midgut cells, to control metabolic function and to enable tissue remodelling. Atg9 is the only transmembrane protein involved in the core autophagy machinery and is thought to have a role in autophagosome formation. During Drosophila development, Atg9 co-located with Atg8 autophagosomes, Rab11 endosomes and Lamp1 endosomes-lysosomes. RNAi silencing of Atg9 reduced both the number and the size of autophagosomes during development and caused morphological changes to amphisomes/autolysosomes. In control cells there was compartmentalised acidification corresponding to intraluminal Rab11/Lamp-1 vesicles, but in Atg9 depleted cells there were no intraluminal vesicles and the acidification was not compartmentalised. We concluded that Atg9 is required to form intraluminal vesicles and for localised acidification within amphisomes/autolysosomes, and consequently when depleted, reduced the capacity to degrade and remodel gut tissue during development.
dc.description.statementofresponsibilityC. A. Bader, T. Shandala, Y. S. Ng, I. R. D. Johnson, D. A. Brooks
dc.identifier.citationBiology Open, 2015; 4(11):1345-1355
dc.identifier.doi10.1242/bio.013979
dc.identifier.issn2046-6390
dc.identifier.issn2046-6390
dc.identifier.orcidBrooks, D. [0000-0001-9098-3626]
dc.identifier.urihttp://hdl.handle.net/2440/99959
dc.language.isoen
dc.publisherCompany of Biologists
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/631915
dc.rights© 2015. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
dc.source.urihttps://doi.org/10.1242/bio.013979
dc.subjectAtg9
dc.subjectautophagy
dc.subjectautophagosome
dc.subjectamphisome
dc.subjectautolysosome
dc.subjectmultivesicular endosome
dc.subjectlysosome
dc.subjectintraluminal vesicles
dc.titleAtg9 is required for intraluminal vesicles in amphisomes and autolysosomes
dc.typeJournal article
pubs.publication-statusPublished

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
hdl_99959.pdf
Size:
1.74 MB
Format:
Adobe Portable Document Format
Description:
Published version
Download

Collections

Molecular and Biomedical Science publications