The CRABS CLAW ortholog from California poppy (Eschscholzia californica, Papaveraceae), EcCRC, is involved in floral meristem termination, gynoecium differentiation and ovule initiation

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dc.contributor.authorOrashakova, S.
dc.contributor.authorLange, M.
dc.contributor.authorLange, S.
dc.contributor.authorWege, S.
dc.contributor.authorBecker, A.
dc.date.issued2009
dc.description.abstractThe Arabidopsis transcription factor CRABS CLAW (CRC) is a major determinant of carpel growth and fusion, and, in concert with other redundantly acting genes, of floral meristem termination. Its rice ortholog, however, has additional functions in specifying carpel organ identity. We were interested in understanding the history of gene function modulation of CRC-like genes during angiosperm evolution. Here, we report the identification and functional characterization of EcCRC, the Californica poppy (Eschscholzia californica) CRC ortholog. The downregulation of EcCRC by virus-induced gene silencing (VIGS) produces additional organ whorls that develop exclusively into gynoecia, resulting in a reiteration of the fourth whorl. Additionally, defects in carpel polarity and ovule initiation are apparent, and the observed phenotype is restricted to the gynoecium. Our results further show that the history of CRC-like genes during angiosperm evolution is characterized by gains of function, independent of duplication processes in this gene subfamily. Moreover, our data indicate that the ancestral angiosperm CRC-like gene was involved in floral meristem termination and the promotion of abaxial cell fate in the gynoecium, and that in the lineage leading to Arabidopsis, additional genes have been recruited to adopt some of these functions, resulting in a high degree of redundancy.
dc.description.statementofresponsibilitySvetlana Orashakova, Matthias Lange, Sabrina Lange, Stefanie Wege, and Annette Becker
dc.identifier.citationThe Plant Journal, 2009; 58(4):682-693
dc.identifier.doi10.1111/j.1365-313X.2009.03807.x
dc.identifier.issn0960-7412
dc.identifier.issn1365-313X
dc.identifier.orcidWege, S. [0000-0002-7232-5889]
dc.identifier.urihttp://hdl.handle.net/2440/88898
dc.language.isoen
dc.publisherWiley
dc.rights© 2009 The Authors
dc.source.urihttps://doi.org/10.1111/j.1365-313x.2009.03807.x
dc.subjectevolutionary developmental genetics; carpel development; YABBY transcription factor; CRABS CLAW; Eschscholzia californica; California poppy
dc.titleThe CRABS CLAW ortholog from California poppy (Eschscholzia californica, Papaveraceae), EcCRC, is involved in floral meristem termination, gynoecium differentiation and ovule initiation
dc.typeJournal article
pubs.publication-statusPublished

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