Method of purifying RNA binding protein-RNA complexes

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Date

2005

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Darnell, R.
Jensen, K.
Ule, J.

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Patent

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Inventors: Darnell Robert, Jensen Kirk, Ule Jernej; Assignee: Darnell Robert, Jensen Kirk, Ule Jernej; Agents: Eitan, Pearl, Latzer & Cohen Zedek LLP

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Abstract

The present invention provides methods for purifying RNA molecules interacting with an RNA binding protein (RBP), and the use of such methods to analyze a gene expression profile of a cell. The invention also provides sequences of RNA molecules that mediate binding to an RBP, proteins encoded by the sequences, a method of identifying the sequences, and the use of the sequences in a screen to identify bioactive molecules. The invention also provides RNA motifs found among the sequences and compounds that bind the RNA motifs. In addition, the invention provides methods of treating diseases associated with a function of an RNA binding protein.

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Priority date Oct 23, 2003 Filing date Jul 27, 2009 Application Date: 25.10.2004 Publication Date: 13.10.2005 Publication number US20110076676 A1 Publication type Application Application number US 12/509,856 Publication date Mar 31, 2011

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