Heterogeneous immunoreactivity of axonal spheroids in focal symmetrical encephalomalacia produced by Clostridium perfringens type D epsilon toxin in sheep
Date
2021
Authors
Manavis, J.
Blumbergs, P.
Jerrett, I.
Hanshaw, D.
Uzal, F.
Finnie, J.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Veterinary pathology, 2021; 59(2):328-332
Statement of Responsibility
Jim Manavis, Peter Blumbergs, Ian Jerrett, Daren Hanshaw, Francisco Uzal, and John Finnie
Conference Name
Abstract
Since axonal injury (AI) is an important component of many veterinary neurologic disorders, we assessed the relative ability of a panel of antibodies (amyloid precursor protein, 3 subunits of neurofilament protein, protein gene product 9.5, ubiquitin, and synaptophysin) to detect axonal swellings or spheroids. Abundant axonal spheroids found in necrotic internal capsule foci produced in 4 sheep by chronic Clostridium perfringens type D epsilon neurotoxicity provided a model system in which to evaluate this important diagnostic tool. There was heterogeneous labeling of subsets of spheroids by the respective antibodies, suggesting that, in order to detect the complete spectrum of AI in diagnostic cases, a range of antibodies should be used, not only when spheroids are plentiful but also when they are few in number or incompletely developed. The application of insufficient markers in the latter cases can potentially lead to the contribution of AI to lesion pathogenesis being underappreciated.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
© The Author(s) 2021