On-column complexation of metal ions using 2,6-pyridinedicarboxylic acid and separation of their anionic complexes by capillary electrophoresis with direct UV detection

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2002

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Chen, Z.
Naidu, R.

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Journal of Chromatography A, 2002; 966(1-2):245-251

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On-column complexation of metal ions with 2,6-pyridinedicarboxylate (2,6-PDC) to form anionic complexes enabled their separation by capillary zone electrophoresis with direct UV detection at 214 nm. Nine metal ions, Cu2+, Zn2+, Ni2+, Cd2+ Mn2+, Pb2+, Fe3+, Al3+ and Ca2+, were determined in less than 7 min using 10 mM 2.6-PDC solution containing 0.75 mM tetradecyltrimethylammonium bromide at pH 4.0. Satisfactory working ranges (20-300 microM), detection limits (3-10 microM) and good repeatability of the peak areas (RSD 2.1-4.2%, n=5) were obtained using hydrodynamic injection (30 s). The proposed method was used successfully for the determination of Mn2+, Fe3+, Al3+ and Ca2+ in groundwaters.

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