Fluorescence recovery after photo-bleaching as a method to determine local diffusion coefficient in the stratum corneum
Date
2012
Authors
Anissimov, Y.G.
Zhao, X.
Roberts, M.S.
Zvyagin, A.V.
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Journal article
Citation
International Journal of Pharmaceutics, 2012; 435(1):93-97
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Abstract
Fluorescence recovery after photo-bleaching experiments were performed in human stratum corneum in vitro. Fluorescence multiphoton tomography was used, which allowed the dimensions of the photobleached volume to be at the micron scale and located fully within the lipid phase of the stratum corneum. Analysis of the fluorescence recovery data with simplified mathematical models yielded the diffusion coefficient of small molecular weight organic fluorescent dye Rhodamine B in the stratum corneum lipid phase of about (3ā6) Ć 10ā9 cm2 sā1. It was concluded that the presented method can be used for detailed analysis of localised diffusion coefficients in the stratum corneum phases for various fluorescent probes.
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Link to a related website: https://research-repository.griffith.edu.au/bitstream/10072/48461/1/82259_1.pdf, Open Access via Unpaywall
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Copyright 2012 Elsevier B.V.