Expression of vacuolar H⁺-pyrophosphatase (OVP3) is under control of an anoxia-inducible promoter in rice

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dc.contributor.authorLiu, Q.
dc.contributor.authorZhang, Q.
dc.contributor.authorBurton, R.
dc.contributor.authorShirley, N.
dc.contributor.authorAtwell, B.
dc.date.issued2010
dc.description.abstractVacuolar H⁺-pyrophosphatase (V-PPase) expression increases in a number of abiotic stresses and is thought to play a role in adaptation to abiotic stresses. This paper reports on the regulation of six V-PPase genes in rice (Oryza sativa L.) coleoptiles under anoxia, using flood tolerant and intolerant cultivars to test our hypothesis. Quantitative PCR analysis showed that one vacuolar H⁺-pyrophosphatase (OVP3) was induced by anoxia, particularly in flood-tolerant rice. Regulation of OVP3 expression under anoxia was investigated by analysing putative OVP promoters. The putative OVP3 promoter contained more previously identified anoxia-inducible motifs than the putative promoters of the other five OVP genes. GUS activity in transgenic rice plants containing the OVP3 promoter region linked to the GUS reporter gene was induced only by anoxia. Salt and cold treatments had little effect on OVP3 promoter-driven GUS expression when compared to the anoxic treatment.
dc.description.statementofresponsibilityQinxiang Liu, Qisen Zhang, Rachel A. Burton, Neil J. Shirley and Brian J. Atwell
dc.identifier.citationPlant Molecular Biology: An International Journal on Molecular Biology, Molecular Genetics and Biochemistry, 2010; 72(1-2):47-60
dc.identifier.doi10.1007/s11103-009-9549-z
dc.identifier.issn0167-4412
dc.identifier.issn1573-5028
dc.identifier.orcidBurton, R. [0000-0002-0638-4709]
dc.identifier.orcidShirley, N. [0000-0001-8114-9891]
dc.identifier.urihttp://hdl.handle.net/2440/59795
dc.language.isoen
dc.publisherKluwer Academic Publ
dc.rights© Springer Science+Business Media B.V. 2009
dc.source.urihttps://doi.org/10.1007/s11103-009-9549-z
dc.subjectAnoxia
dc.subjectGene expression
dc.subjectPromoter
dc.subjectRice
dc.subjectVacuolar H⁺-pyrophosphatase
dc.titleExpression of vacuolar H⁺-pyrophosphatase (OVP3) is under control of an anoxia-inducible promoter in rice
dc.title.alternativeExpression of vacuolar H(+)-pyrophosphatase (OVP3) is under control of an anoxia-inducible promoter in rice
dc.typeJournal article
pubs.publication-statusPublished

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