A pneumococcal MerR-like regulator and S-nitrosoglutathione reductase are required for systemic virulence
Date
2007
Authors
Stroeher, U.
Kidd, S.
Stafford, S.
Jennings, M.
Paton, J.
McEwan, A.
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Journal article
Citation
Journal of Infectious Diseases, 2007; 196(12):1820-1826
Statement of Responsibility
Uwe H. Stroeher, Robert S. Kidd, Sian L. Stafford, Michael P. Jennings, James C. Paton and Alastair G. McEwan
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Abstract
A transcriptional regulator, NmlR(sp), has been identified in Streptococcus pneumoniae that is required for defense against nitric oxide (NO) stress. The nmlR(sp) gene is cotranscribed with adhC, which encodes an alcohol dehydrogenase that is able to reduce S-nitrosoglutathione (GSNO) with NADH as reductant. nmlR(sp) and adhC mutants exhibited a reduced level of NADH-GSNO oxidoreductase activity and were more susceptible to killing by NO than were wild-type cells. Comparison of the virulence of wild-type and mutant strains by use of a mouse model system showed that NmlR(sp) and AdhC do not play a key role in the adherence of pneumococci to the nasopharynx in vivo. An intraperitoneal challenge experiment revealed that both NmlR(sp) and AdhC were required for survival in blood. These data identify novel components of a NO defense system in pneumococci that are required for systemic infection.
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Copyright © 2007 by the Infectious Diseases Society of America. All rights reserved.