Building a plant DNA barcode reference library for a diverse tropical flora: an example from Queensland, Australia
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Date
2016
Authors
Costion, C.
Lowe, A.
Rossetto, M.
Kooyman, R.
Breed, M.
Ford, A.
Crayn, D.
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Diversity, 2016; 8(1):5-1-5-9
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Craig M. Costion, Andrew J. Lowe, Maurizio Rossetto, Robert M. Kooyman, Martin F. Breed, Andrew Ford and Darren M. Crayn
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Abstract
A foundation for a DNA barcode reference library for the tropical plants of Australia is presented here. A total of 1572 DNA barcode sequences are compiled from 848 tropical Queensland species. The dataset represents 35% of the total flora of Queensland’s Wet Tropics Bioregion, 57% of its tree species and 28% of the shrub species. For approximately half of the sampled species, we investigated the occurrence of infraspecific molecular variation in DNA barcode loci rbcLa, matK, and the trnH-psbA intergenic spacer region across previously recognized biogeographic barriers. We found preliminary support for the notion that DNA barcode reference libraries can be used as a tool for inferring biogeographic patterns at regional scales. It is expected that this dataset will find applications in taxonomic, ecological, and applied conservation research.
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© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).