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The multivesicular body-localized GTPase ARFA1b/1c is important for callose deposition and ROR2 syntaxin-dependent preinvasive basal defense in barley

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dc.contributor.authorBohlenius, H.
dc.contributor.authorMorch, S.
dc.contributor.authorGodfrey, D.
dc.contributor.authorNielsen, M.
dc.contributor.authorThordal-Christensen, H.
dc.date.issued2010
dc.description.abstractHost cell vesicle traffic is essential for the interplay between plants and microbes. ADP-ribosylation factor (ARF) GTPases are required for vesicle budding, and we studied the role of these enzymes to identify important vesicle transport pathways in the plant–powdery mildew interaction. A combination of transient-induced gene silencing and transient expression of inactive forms of ARF GTPases provided evidence that barley (Hordeum vulgare) ARFA1b/1c function is important for preinvasive penetration resistance against powdery mildew, manifested by formation of a cell wall apposition, named a papilla. Mutant studies indicated that the plasma membrane–localized REQUIRED FOR MLO-SPECIFIED RESISTANCE2 (ROR2) syntaxin, also important for penetration resistance, and ARFA1b/1c function in the same vesicle transport pathway. This was substantiated by a requirement of ARFA1b/1c for ROR2 accumulation in the papilla. ARFA1b/1c is localized to multivesicular bodies, providing a functional link between ROR2 and these organelles in penetration resistance. During Blumeria graminis f sp hordei penetration attempts, ARFA1b/1c-positive multivesicular bodies assemble near the penetration site hours prior to the earliest detection of callose in papillae. Moreover, we showed that ARFA1b/1c is required for callose deposition in papillae and that the papilla structure is established independently of ARFA1b/1c. This raises the possibility that callose is loaded into papillae via multivesicular bodies, rather than being synthesized directly into this cell wall apposition.
dc.description.statementofresponsibilityHenrik Böhlenius, Sara M. Mørch, Dale Godfrey, Mads E. Nielsen and Hans Thordal-Christensen
dc.identifier.citationThe Plant Cell, 2010; 22(11):3831-3844
dc.identifier.doi10.1105/tpc.110.078063
dc.identifier.issn1040-4651
dc.identifier.issn1532-298X
dc.identifier.urihttp://hdl.handle.net/2440/62885
dc.language.isoen
dc.publisherAmer Soc Plant Physiologists
dc.rights© 2010 American Society of Plant Biologists
dc.source.urihttps://doi.org/10.1105/tpc.110.078063
dc.subjectEndosomes
dc.subjectHordeum
dc.subjectPlant Leaves
dc.subjectGlucans
dc.subjectADP-Ribosylation Factors
dc.subjectrab GTP-Binding Proteins
dc.subjectPlant Proteins
dc.subjectArabidopsis Proteins
dc.subjectRecombinant Fusion Proteins
dc.subjectPhylogeny
dc.subjectPlant Diseases
dc.subjectGene Silencing
dc.subjectBiological Transport
dc.subjectQa-SNARE Proteins
dc.subjectReceptor Tyrosine Kinase-like Orphan Receptors
dc.subjectMultivesicular Bodies
dc.titleThe multivesicular body-localized GTPase ARFA1b/1c is important for callose deposition and ROR2 syntaxin-dependent preinvasive basal defense in barley
dc.typeJournal article
pubs.publication-statusPublished

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