Optical fiber DNA biosensor with temperature monitoring based on double microcavities Fabry-Perot interference and Vernier combined effect
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Date
2023
Authors
Li, X.
Li, F.
Zhou, X.
Zhang, Y.
Nguyen, L.V.
Warren Smith, S.C.
Zhao, Y.
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IEEE Transactions on Instrumentation and Measurement, 2023; 72(7002108):1-8
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Abstract
This paper introduces a label-free DNA sensor based on the based on double microcavities Fabry-Perot interference and Vernier effect. The sensor can simultaneously monitor temperature to avoid interference with the process of measuring DNA. The biosensor is composed of two open microcavities made of C-type optical fiber. One of the microcavities will is functionalized by the probe DNA (pDNA) to hybridize with complementary DNA (cDNA). And the other microcavity is filled with PDMS to realize temperature sensitivity.
At the same time, the two cavities will form a Vernier effect, which is sensitive to both temperature and DNA. It is proved by experiments that the temperature sensitivity and refractive index (RI) sensitivity of the sensor are -2.79 nm/°C and -7122.63 nm/RIU, respectively. The proposed sensor has good specificity. cDNA sequence can bind well to given targets, but hardly bind to non-complementary DNA (non-cDNA) sequences. Moreover, the sensor offers some other has the benefits of high sensitivity, dual-parameter detection, real-time response, and simple fabrication. This is of great significance to solve the problem of temperature sensitivity of biosensors.
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Copyright 2022 IEEE
Access Condition Notes: Accepted manuscript available after 1 January 2025