EphB/ephrin-B interaction mediates adult stem cell attachment spreading and migration: Implications for dental tissue repair
Date
2007
Authors
Arthur, A.
Shi, S.
Sun, T.
Bartold, P.
Koblar, S.
Gronthos, S.
Editors
Advisors
Journal Title
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Volume Title
Type:
Journal article
Citation
Stem Cells, 2007; 25(1):156-164
Statement of Responsibility
Agnieszka Stokowski, Songtao Shi, Tao Sun, Peter Mark Bartold, Simon Andrea Koblar, Stan Gronthos
Conference Name
Abstract
Human adult dental pulp stem cells (DPSCs) reside predominantly within the perivascular niche of dental pulp and are thought to originate from migrating neural crest cells during development. The Eph family of receptor tyrosine kinases and their ligands, the ephrin molecules, play an essential role in the migration of neural crest cells during development and stem cell niche maintenance. The present study examined the expression and function of the B-subclass Eph/ephrin molecules on DPSCs. Multiple receptors were primarily identified on DPSCs within the perivascular niche, whereas ephrin-B1 and ephrin-B3 were expressed by the surrounding pulp tissue. EphB/ephrin-B bidirectional signaling inhibited cell attachment and spreading, predominately via the mitogen-activated protein kinase (MAPK) pathway for forward signaling and phosphorylation of Src family tyrosine kinases via reverse ephrin-B signaling. DPSC migration was restricted through unidirectional ephrin-B1-activated EphB forward signaling, primarily signaling through the MAPK pathway. Furthermore, we observed that ephrin-B1 was downregulated in diseased adult teeth compared with paired uninjured controls. Collectively, these studies suggest that EphB/ephrin-B molecules play a role in restricting DPSC attachment and migration to maintain DPSCs within their stem cell niche under steady-state conditions. These results may have implications for dental pulp development and regeneration.
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Dissertation Note
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Copyright © 2007 AlphaMed Press