Development of an mlrA gene-directed TaqMan PCR assay for quantitative assessment of microcystin-degrading bacteria within water treatment plant sand filter biofilms

Date

2009

Authors

Hoefel, D.
Adriansen, C.
Bouyssou, M.
Saint, C.
Newcombe, G.
Ho, L.

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Journal article

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Applied and Environmental Microbiology, 2009; 75(15):5167-5169

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Daniel Hoefel, Caroline M. M. Adriansen, Magali A. C. Bouyssou, Christopher P. Saint, Gayle Newcombe and Lionel Ho

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Abstract

We report for the first time a quantitative mlrA gene-directed TaqMan PCR assay for the rapid detection of microcystin-degrading bacteria. This was applied, in combination with 16S ribosomal DNA-directed quantitative PCR and denaturing gradient gel electrophoresis, to study virgin sand filter column biofilm development and to correlate mlrA gene abundance with microcystin removal efficiency.

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Copyright © 2009, American Society for Microbiology. All Rights Reserved.

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Copyright 2009 American Society for Microbiology

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