A DNA-based method for studying root responses to drought in field-grown wheat genotypes
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(Published version)
Date
2013
Authors
Huang, C.
Kuchel, H.
Edwards, J.
Hall, S.
Parent, B.
Eckermann, P.
Herdinda, X.
Hartley, D.
Langridge, P.
McKay, A.
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Journal article
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Scientific Reports, 2013; 3(1):1-7
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Chun Y. Huang, Haydn Kuchel, James Edwards, Sharla Hall, Boris Parent, Paul Eckermann, Herdina, Diana M. Hartley, Peter Langridge & Alan C. McKay
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Abstract
Root systems are critical for water and nutrient acquisition by crops. Current methods measuring root biomass and length are slow and labour-intensive for studying root responses to environmental stresses in the field. Here, we report the development of a method that measures changes in the root DNA concentration in soil and detects root responses to drought in controlled environment and field trials. To allow comparison of soil DNA concentrations from different wheat genotypes, we also developed a procedure for correcting genotypic differences in the copy number of the target DNA sequence. The new method eliminates the need for separation of roots from soil and permits large-scale phenotyping of root responses to drought or other environmental and disease stresses in the field.
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This work is licensed under a Creative Commons Attribution- NonCommercial-NoDerivs 3.0 Unported license. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0