Insulin receptor expression in primary and cultured osteoclast-like cells
Date
1998
Authors
Thomas, D.
Udagawa, N.
Hards, D.
Quinn, J.
Moseley, J.
Findlay, D.
Best, J.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Bone, 1998; 23(3):181-186
Statement of Responsibility
Thomas, D.M. ; Udagawa, N. ; Hards, D.K. ; Quinn, J.M.W. ; Moseley, J.M. ; Findlay, D.M. ; Best, J.D.
Conference Name
Abstract
Skeletal growth is the net product of coordinated bone formation and resorption. Insulin is known to stimulate bone formation by actions on osteoblasts. It is not known whether insulin receptors are present on osteoclasts, or whether insulin regulates osteoclastic function. We present here immunocytochemical evidence of insulin receptor expression by mature mono- and multinucleated murine osteoclast-like cells generated in vitro, and in primary neonatal rat and mouse osteoclasts. Radiolabeled studies indicated that progressive enrichment of osteoclast-like cells in coculture was associated with increased insulin binding. When osteoclast-like cells generated in vitro were plated onto dentine slices, insulin dose-dependently inhibited pit formation by up to 80%, suggesting a role for insulin in osteoclast function. These data are consistent with an effect of insulin on bone resorption in addition to those previously recognized on bone formation, actions that together result in net bone growth.