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Association of Helicobacter pylori with colorectal cancer development

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dc.contributor.authorAlsamak, F.
dc.contributor.authorAbdulamir, A.
dc.contributor.authorMahdi, L.
dc.contributor.authorAlnaib, K.
dc.contributor.authorBakar, F.
dc.date.issued2010
dc.description.abstractBackground: Helicobacter pylori (H. pylori) may be associated with colorectal cancer. However, the underlying mechanisms are still unclear. Objectives: Explore the serostatus of H. pylori cytotoxicity-associated gene A product (CagA) in patients with colorectal carcinoma, and assess the association of H. pylori with colorectal cancer via c-Myc and MUC-2 proteins at tumor tissues. Methods: H. pylori CagA IgG antibodies were screened using enzyme-linked immunosorbent assay (ELISA) in 30 patients with colorectal carcinoma and 30 cancer-free control subjects. Paraffin-embedded blocks were examined for the expression of c-Myc and MUC-2 protein by immunohistochemistry. Results: H. pylori CagA seropositivity increased significantly among colorectal cancer patients (p <0.05). The expression of c-Myc and MUC-2 in colorectal carcinoma patients was over-expressed (80%), and downexpressed (63%) in resection margins (p <0.05). c-Myc over-expression and MUC-2 down-expression were associated with CagA-positive rather than CagA-negative H. pylori patients. In 16 CagA seropositive vs. 14 CagA seronegative patients, the expression rate was 97.3% vs. 64.2% and 33.3% vs. 78.5% for cMyc and MUC-2, respectively. CagA IgG level was significantly higher in positive than in negative c-Myc patients (p= 0.036), and in negative than in positive MUC-2 patients (p= 0.044). c-Myc and MUC-2 were positively and inversely correlated with CagA IgG level (p <0.05). Conclusions: CagA-seropositive H. pylori is most probably associated with colorectal cancer development. Part of the underlying mechanism for such association might be via alterations in expression of MUC-2, which depletes the mucous protective layer in the colo-rectum, and c-Myc, which stimulates the growth of cancerous cells.
dc.description.statementofresponsibilityFadi Fouad Alsamak, Ahmed Sahib Abdulamir, Laila Khalid Mahdi, Khalid Alnaib and Fatimah Abu Bakar
dc.description.urihttp://www.asianbiomed.org/AutoJournal.php
dc.identifier.citationAsian Biomedicine, 2010; 4(4):609-618
dc.identifier.doi10.2478/abm-2010-0077
dc.identifier.issn1905-7415
dc.identifier.issn1875-855X
dc.identifier.orcidMahdi, L. [0000-0002-5878-8385]
dc.identifier.urihttp://hdl.handle.net/2440/62094
dc.language.isoen
dc.publisherChulalongkorn University
dc.rightsCopyright status unknown
dc.source.urihttp://www.asianbiomed.org/htdocs/previous/A20104609.pdf
dc.subjectHelicobacter pylori
dc.subjectcolorectal cancer
dc.subjectCag-A
dc.subjectMUC-2
dc.titleAssociation of Helicobacter pylori with colorectal cancer development
dc.typeJournal article
pubs.publication-statusPublished

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