Fitch, A.Leeworthy, G.Li, X.Bowman, W.Turner, L.Gardner, M.2015-05-172015-05-172012Australian Journal of Zoology, 2012; 60(6):368-3710004-959X1446-5698http://hdl.handle.net/2440/91135We isolated 18 new, polymorphic, microsatellite markers from Holothuria scabra, a commercially important species of sea cucumber found throughout the Asia-Pacific region. Next-generation sequencing was used to identify 206 unique loci for which primers were designed. Of these unique loci, we trialled 65 primer pairs in the target species, and 48 (74%) amplified a product of the expected size. Eighteen loci were found to be polymorphic and reliable, and were screened for variation in 50 individuals, from a single population from Croker Island, Northern Territory, Australia. Observed heterozygosity ranged from 0.00 to 0.96 (mean = 0.46) and the number of alleles per locus from 2 to 28 (mean = 9.61). These loci will be useful for the investigation of population structure and mating systems in H. scabra and may also be of use in other holothurian species.enCopyright the authorsGS-FLX; next-generation sequencingJournal article003000907710.1071/ZO121140003196813000022-s2.0-8487856023788824Li, X. [0000-0001-8510-624X]