Worapamorn, W.Haase, H.Li, H.Bartold, P.2007-12-202007-12-202001Journal of Cellular Physiology, 2001; 186(3):448-4560021-95411097-4652http://hdl.handle.net/2440/40046Published online in Wiley InterScience, 23 January 2001Cell-surface proteoglycans have been known to be involved in many functions including interactions with components of the extracellular microenvironment, and act as co-receptors which bind and modify the action of various growth factors and cytokines. The purpose of this study was to determine the regulation by growth factors and cytokines on cell-surface proteoglycan gene expression in cultured human periodontal ligament (PDL) cells. Subconfluent, quiescent PDL cells were treated with various concentrations of serum, bFGF, PDGF-BB, TGF-beta1, IL-beta1, and IFN-gamma. RT-PCR technique was used, complemented with Northern blot for syndecan-1, to examine the effects of these agents on the mRNA expression of five cell-surface proteoglycans (syndecan-1, syndecan-2, syndecan-4, glypican and betaglycan). Syndecan-1 mRNA levels increased in response to serum, bFGF and PDGF-BB, but decreased in response to TGF-beta1, IL-1beta and IFN-gamma. In contrast, syndecan-2 mRNA levels were upregulated by TGF-beta1 and IL-1beta stimulation, but remained unchanged with the other agents. Betaglycan gene expression decreased in response to serum, but was upregulated by TGF-beta1 and unchanged by the other stimulants. Additionally, syndecan-4 and glypican were not significantly altered in response to the regulator molecules studied, with the exception that glypican is decreased in response to IFN-gamma. These data demonstrate that the gene expression of the five cell-surface proteoglycans studied is differentially regulated in PDL cells lending support to the notion of distinct functions for these cell-surface proteoglycans.en© 2001 Wiley-Liss, Inc.Cells, CulturedCell MembranePeriodontal LigamentHumansProteoglycansGrowth SubstancesFibroblast Growth Factor 2Platelet-Derived Growth FactorProto-Oncogene Proteins c-sisTransforming Growth Factor betabeta 2-MicroglobulinMembrane GlycoproteinsReceptors, Transforming Growth Factor betaRecombinant ProteinsInterleukin-1CytokinesReverse Transcriptase Polymerase Chain ReactionCell DivisionCell MovementTranscription, GeneticGene Expression RegulationHeparan Sulfate ProteoglycansSyndecan-1SyndecansSyndecan-4Syndecan-2Interferon-gammaBecaplerminJournal article00200649482007122010011410.1002/1097-4652(2001)9999:9999<000::AID-JCP1047>3.0.CO;2-V0001667190000152-s2.0-003512869150023Bartold, P. [0000-0002-5695-3877] [0000-0002-6225-3084]