Kalionis, B.Dodd, I.B.Egan, J.B.2014-11-202014-11-201986Journal of Molecular Biology, 1986; 191(2):199-2090022-28361089-8638http://hdl.handle.net/2440/87412The PstI fragment (65.5% to 77.4%) of coliphage 186, known genetically to encode the major control genes, has been sequenced, and an analysis performed to assess coding capacity, transcription-translation signals, and to identify any other significant features. Our analysis indicates that the region encodes: (1) seven genes, including the int and cI genes, which overlap, the late control gene B, and two genes, named CP75 and CP76, encoding potential DNA-binding proteins; (2) a promoter pB and terminator tB for the rightward transcription of the B gene, and we predict the existence of this transcript in a lysogen; (3) a promoter pL and terminator tL for leftward transcription that encodes the int and cI genes, and represents the presumed lysogenic transcript; (4) a promoter pR for rightward transcription to give the presumed (early) lytic transcript that is overlapping and convergent with the lysogenic transcript; and finally (5), a potential operator site for repressor binding in the region of the pR promoter. Preliminary evidence is presented to support this analysis.en© 1986 Academic Press, Inc.RibosomesColiphagesDNA, ViralTranscription, GeneticGene Expression RegulationBinding SitesBase SequenceRepetitive Sequences, Nucleic AcidPromoter Regions, GeneticTerminator Regions, GeneticJournal article003001313010.1016/0022-2836(86)90257-3A1986E2282000062-s2.0-0022551411152005Dodd, I.B. [0000-0003-2969-6841]