Chan, H.Y.Schjenken, J.E.Sharkey, D.J.Robertson, S.A.Álvarez-Rodríguez, M.2025-12-012025-12-012025Spermatology: Methods and Protocols, 2025 / Álvarez-Rodríguez, M. (ed./s), vol.2897, Ch.44, pp.649-662978-1-0716-4406-5https://hdl.handle.net/2440/148365Series Editor John M. Walker, Print ISSN 1064-3745 ; Electronic ISSN 1940-6029.After intromission of seminal fluid at mating in mammals, the seminal fluid interacts with the lining of the female reproductive tract and elicits a female immune response. This response is triggered by signals in seminal fluid (carried by both the sperm and seminal plasma components) that bind receptors on female tract epithelial cells, causing a change in their transcriptional program to upregulate expression of pro-inflammatory cytokines and chemokines. The ensuing leukocyte recruitment and immune activation facilitates conception, embryo development, and progression of pregnancy. In this chapter, we report methods for evaluating effects of seminal fluid and its components (seminal plasma and sperm) on the female reproductive tract immune response in mice.en© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025Seminal fluid; Seminal plasma; Sperm; Uterus; Cervix; Inflammation; MiceGenitalia, FemaleSpermatozoaSemenAnimalsMicePregnancyFemaleMaleBook chapter10.1007/978-1-0716-4406-5_44736951Schjenken, J.E. [0000-0001-6293-6160]Sharkey, D.J. [0000-0002-4831-7950]Robertson, S.A. [0000-0002-9967-0084]