Paton, J.Morona, R.Paton, A.A.P.T. Patent and Trade Mark Attorneys2013-01-032013-01-032010http://hdl.handle.net/2440/74712PATENT: A. W. Paton, J. C. Paton (Assignee Adelaide Research and Innovation Pty Ltd). AU 2004/201978 A1 filed May 12, 2004; published Jul 7, 2005; Standard Patent Sealed Jun 24, 2010; expiry date May 12, 2024.A member of a new class of bacterial toxin has been isolated and characterised. The bacterial toxin is of the AB₅ type and is characterised in that it has a subtilase domain. It is cytotoxic to Vero cells, and toxicity in vivo in mice occurs in a number of different sites. Mutation of the serine active residue results in greater than 99% reduction in activity. The protein has been purified and antibodies have been prepared for both the A subunit and B subunits, and ELISA detection methods have been developed. The nucleic acid sequence has been determined and primers specific for the toxin have been used for a preliminary screen of a range of patient samples to ascertain the extent to which the toxin is present.enAU 2004/201978 B2Patent0020114407200420197826993Paton, J. [0000-0001-9807-5278]Morona, R. [0000-0001-7009-7440]