Ainsley, P.Hammerschlag, F.Bertozzi, T.Collins, G.Sedgley, M.2006-07-212006-07-212001Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants, 2001; 67(3):221-2260167-6857http://hdl.handle.net/2440/13093The original publication can be found at www.springerlink.comAdventitious shoots were regenerated from immature cotyledons of four almond cultivars (`Ne Plus Ultra', `Nonpareil', `Carmel' and `Parkinson'). Open-pollinated fruit were collected from orchard-grown trees 100–115 days after full bloom. The proximal ends of the cotyledons were excised and the embryonic axes discarded. The effects of different concentrations of thidiazuron (TDZ) and indole-3-butyric acid (IBA) and the presence or absence of light for the first 7 days of culture were determined. Shoot regeneration rates were highest for cotyledons cultured for 8 weeks on Murashige and Skoog (MS) basal medium containing TDZ (10.0 mgrM), followed by 4 weeks on medium without plant growth regulators. Regeneration levels were further improved if cotyledons were maintained in darkness for the first 7 days. IBA (0.5 mgrM) significantly reduced the development of adventitious shoots. The frequency of cotyledons that developed adventitious shoots under the optimum tested conditions for `Ne Plus Ultra', `Nonpareil', `Carmel', and `Parkinson' were 80.0%, 73.3%, 100.0% and 86.7%, respectively.en© 2001 Kluwer Academic Publishersadventitious shootsindole-3-butyric acidorganogenesisPrunus dulcis MillthidiazuronJournal article002001009310.1023/A:10127007140850001720740000022-s2.0-003566438262217Bertozzi, T. [0000-0001-6665-3395]